Assessment of drug-induced mitochondrial dysfunction via altered cellular respiration and acidification measured in a 96-well platform.

@article{Nadanaciva2012AssessmentOD,
  title={Assessment of drug-induced mitochondrial dysfunction via altered cellular respiration and acidification measured in a 96-well platform.},
  author={Sashi Nadanaciva and Payal Rana and Gyda C Beeson and Denise A. Chen and David A. Ferrick and Craig Cano Beeson and Yvonne Will},
  journal={Journal of bioenergetics and biomembranes},
  year={2012},
  volume={44 4},
  pages={421-37}
}
High-throughput applicable screens for identifying drug-induced mitochondrial impairment are necessary in the pharmaceutical industry. Hence, we evaluated the XF96 Extracellular Flux Analyzer, a 96-well platform that measures changes in the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of cells. The sensitivity of the platform was bench-marked with known modulators of oxidative phosphorylation and glycolysis. Sixteen therapeutic agents were screened in HepG2 cells… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 1 time over the past 90 days. VIEW TWEETS

Citations

Publications citing this paper.
Showing 1-10 of 24 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 45 references

Structural analysis of ATP synthase from bovine heart mitochondria

  • JE Walker, IR Collinson, MJ Van Raaij, MJ Runswick
  • Methods Enzymol
  • 1995
Highly Influential
2 Excerpts

Similar Papers

Loading similar papers…