Assessment of circulating 25(OH)D and 1,25(OH)2D: emergence as clinically important diagnostic tools.

@article{Hollis2007AssessmentOC,
  title={Assessment of circulating 25(OH)D and 1,25(OH)2D: emergence as clinically important diagnostic tools.},
  author={Bruce W. Hollis},
  journal={Nutrition reviews},
  year={2007},
  volume={65 8 Pt 2},
  pages={S87-90}
}
One of the major factors responsible for the explosion of knowledge related to vitamin D metabolism and its relation to clinical disease was the introduction of competitive protein-binding assays for 25(OH)D and 1,25(OH)2D. 2 These assays were introduced more than three decades ago, and were based on assessing circulating 25(OH)D or 1,25(OH)2D using the vitamin D-binding protein or chick intestinal receptor, respectively, as the primary binding agents. Both of these assays used H-labeled… CONTINUE READING

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