Assessment of cell viability.

  title={Assessment of cell viability.},
  author={Simon Johnson and Vy Nguyen and David M. Coder},
  journal={Current protocols in cytometry},
  volume={Chapter 9},
Cell viability may be judged by morphological changes or by changes in membrane permeability and/or physiological state inferred from the exclusion of certain dyes or the uptake and retention of others. This unit presents methods based on dye exclusion, esterase activity, and mitochondrial membrane potential, as well as protocols for determining the pre-fixation viability of fixed cells either before or after fixation with amine-reactive dyes suitable for a range of excitation wavelengths… CONTINUE READING

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