Assessment of cell viability in three-dimensional scaffolds using cellular auto-fluorescence.

@article{Dittmar2012AssessmentOC,
  title={Assessment of cell viability in three-dimensional scaffolds using cellular auto-fluorescence.},
  author={Roman Dittmar and Esther Potier and Marc A. M. J. van Zandvoort and Keita Ito},
  journal={Tissue engineering. Part C, Methods},
  year={2012},
  volume={18 3},
  pages={198-204}
}
After assessing cell viability (CV), tissue-engineered constructs are often discarded, as current CV assays commonly require specific (fluorescent) dyes to stain cells and may need scaffold/tissue digestion before quantifying the live and dead cells. Here, we demonstrate and evaluate how cellular auto-fluorescence can be exploited to facilitate a noninvasive CV estimation in three-dimensional scaffolds using two advanced microscopy methods. Mixtures of live and dead C2C12 myoblasts (0%, 25%, 50… CONTINUE READING