Assessment of acetone as an alternative to acetonitrile in peptide analysis by liquid chromatography/mass spectrometry.

@article{Fritz2009AssessmentOA,
  title={Assessment of acetone as an alternative to acetonitrile in peptide analysis by liquid chromatography/mass spectrometry.},
  author={Ria Fritz and Wolfgang Ruth and Udo Kragl},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2009},
  volume={23 14},
  pages={2139-45}
}
Acetonitrile as a solvent used in liquid chromatography/mass spectrometry (LC/MS) of peptides and proteins is a relatively toxic solvent (LD50 oral; rat; 2,460 mg/kg) compared to alternatives like methanol (LD50 oral; rat; 5,628 mg/kg) and acetone (LD50 oral; rat; 5,800 mg/kg). Strategies to minimize its consumption in LC are either to reduce the inner diameter of the column or replace acetonitrile with a suitable alternative. Methanol is often recommended to replace acetonitrile in peptide… CONTINUE READING