Assessment of N-glycan heterogeneity of cactus glycoproteins by one-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

@article{Balen2007AssessmentON,
  title={Assessment of N-glycan heterogeneity of cactus glycoproteins by one-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.},
  author={Biljana Balen and Marijana Krsnik-Rasol and Alina D Zamfir and Ivana Zadro and Sergey Y. Vakhrushev and Jasna Peter-Katalini{\'c}},
  journal={Journal of biomolecular techniques : JBT},
  year={2007},
  volume={18 3},
  pages={162-72}
}
Artificial environmental conditions in tissue culture, such as elevated relative humidity and rich nutrient medium, can influence and modify tissue growth and induce spontaneous changes from characteristic organization pattern to unorganized callus. As succulent plants with crassulacean acid metabolism, cacti are particularly susceptible to this altered growth environment. Glycosylated proteins of Mammillaria gracillis tissues cultivated in vitro, separated by SDS-PAGE, were detected with Con A… CONTINUE READING