Assessment of Analgesic and Antibacterial Activity of Premna Integrifolia Linn. (family: Verbenaceae) Leaves


Premna integrifolia Linn. (Family: Verbenaceae) is a medicinal plant which is traditionally used against a number of diseases including inflammatory condition. On the scientific basis of traditional uses, the ethanolic extract of leaves of Premna integrifolia Linn. was assessed for its possible analgesic and antimicrobial activity. The analgesic activity of the sample was studied using acetic acid induced writhing model in mice. Antibacterial activity of leaves of Premna integrifolia Linn. was tested by using the disc diffusion method. In analgesic activity, the extract produced 52.17% (p<0.01) acetic acid induced writhing inhibition in mice at the dose of 500 mg/kg body weight, which is comparable to diclofenac sodium 65.21% (p<0.01) at the dose of 25 mg/kg body weight. The extract showed significant antibacterial activity against both gram positive and gram negative bacteria. All the results tend to justify the traditional uses of the plant and require further investigation to identify the chemicals responsible for these effects. INTRODUCTION: Premna integrifolia Linn. is a large shrub or a small tree with sub-cordate-ovate, opposite leaves, small greenish or yellowish white flowers in cymes, grows naturally and commonly planted in Sundarban and Chittagong. It is commonly known as ganiari, gambari, bhutbirabi . Stem bark contains tannin, bitter alkaloids, premnine, ganiarine and ganikarine, reducing sugars and unsaturated hydrocarbons. Leaves contain verbascoside, vervascoside iridoid glucoside conjugate, premcoryoside and three monoacyl-6-O-alpha-Lrhamnopyranosylcatalpols . Two new phenolic diterpenoids have been isolated from the root bark . Decoction of root is cordial, stomachic, carminative, alterative, tonic and good for liver complaints, and also useful in urticaria. Leaves are used in fever, colic, flatulence, gonorrhea, convulsion, neuralgia and rheumatism. Extract of stem bark decreases force of contraction of heart and produces dilation of pupils . MATERIALS AND METHODS: Sample collection and extraction: The leaves of Premna integrifolia Linn. were collected from the road side area of Dinajpur, Bangladesh in June, 2009 and identified by the experts of Bangladesh National Herbarium, Mirpur, Dhaka (Accession No. DACB34546). A voucher specimen has been deposited in Pharmacy Discipline, Khulna University, Khulna9208,

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@inproceedings{Karmakar2011AssessmentOA, title={Assessment of Analgesic and Antibacterial Activity of Premna Integrifolia Linn. (family: Verbenaceae) Leaves}, author={Utpal Kumar Karmakar and Soma Pramanik and Samir Kumar Sadhu and Manik Chandra Shill and Subrata Kumar}, year={2011} }