Assessment by a fluorochrome microassay of phagocytic killing of group B streptococci adherent to glass.

@article{Rainard1986AssessmentBA,
  title={Assessment by a fluorochrome microassay of phagocytic killing of group B streptococci adherent to glass.},
  author={Pascal Rainard},
  journal={Journal of immunological methods},
  year={1986},
  volume={94 1-2},
  pages={
          113-8
        }
}
An in vitro adherence assay was developed to study the interaction between bovine polymorphonuclear cells (PMN) and group B streptococci (GBS) on glass. The kinetics of uptake and killing of GBS in the presence and absence of opsonins were compared by staining bacteria with a fluorochrome (acridine orange). Opsonization was not required for the PMN to ingest and kill glass-adherent GBS. Nevertheless, the presence of serum during the phagocytosis incubation, or preopsonization of bacteria, both… CONTINUE READING