Assessing the viability of a clumpy mnn9 strain of Saccharomyces cerevisiae used in the manufacture of recombinant pharmaceutical proteins

@article{Duncan2004AssessingTV,
  title={Assessing the viability of a clumpy mnn9 strain of Saccharomyces cerevisiae used in the manufacture of recombinant pharmaceutical proteins},
  author={Paul A. Duncan and Se{\'a}n Gallagher and Linda J. McKerral and P. K. Tsai},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={2004},
  volume={31},
  pages={500-506}
}
Demonstration of the viability of cryopreserved cell bank used to make a biopharmaceutical product is an important indicator of the ability to consistently manufacture over a long period of time, and is mandated in regulatory guidances. A mnn9 strain of Saccharomyces cerevisiae, chosen for its inability to hypermannosylate vaccine antigens, has a clumpy growth tendency due to the inactivation of the gene MNN9 (wild-type), complicating the interpretation of conventional viability measurements… 
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