Assessing the survival of transgenic plant DNA in the human gastrointestinal tract

  title={Assessing the survival of transgenic plant DNA in the human gastrointestinal tract},
  author={Trudy Netherwood and Susana Mar{\'i}a Mart{\'i}n-Or{\'u}e and Anthony G. O'donnell and Sally Gockling and Julia Graham and John Cummings Mathers and Harry J. Gilbert},
  journal={Nature Biotechnology},
The inclusion of genetically modified (GM) plants in the human diet has raised concerns about the possible transfer of transgenes from GM plants to intestinal microflora and enterocytes. The persistence in the human gut of DNA from dietary GM plants is unknown. Here we study the survival of the transgene epsps from GM soya in the small intestine of human ileostomists (i.e., individuals in which the terminal ileum is resected and digesta are diverted from the body via a stoma to a colostomy bag… 

The Stability and Degradation of Dietary DNA in the Gastrointestinal Tract of Mammals: Implications for Horizontal Gene Transfer and the Biosafety of GMOs

Despite the ability of several bacterial species to acquire external DNA by natural transformation, in vivo transfer of dietary DNA to bacteria in the intestine has not been detected in the few experimental studies conducted so far.

Assessing the Transfer of Genetically Modified DNA from Feed to Animal Tissues

It is unlikely that the occurrence of genetic transfer associated with GM plants is higher than that from conventional plants, but DNA transfer may occur independently from the source and the type of the gene.

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

In an in vivo experiment conducted on laboratory rats with the use of transgenic cucumbers expressing the pre-prothaumatin gene, the presence oftransgenic DNA in the tissue of kidneys and liver was not detected and resistance to neomycin of gastrointestinal tract microflora of the rats fed the GMO diet was not found.

Addressing the issue of horizontal gene transfer from a diet containing genetically modified components into rat tissues

The present work was conducted to evaluate the possibility of horizontal gene transfer from a diet containing DNA segments from Cauliflower mosaic virus -35S promoter (CaMVP-35S) to the cells of different organs of rats fed for three months on diets containing genetically modified components.

Addressing concerns over the fate of DNA derived from genetically modified food in the human body: A review.

  • M. NawazR. Mesnage G. Chung
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2019

Horizontal transfer of antibiotic resistance genes into microflora and blood cells in rats fed on GM-diet

The results unambiguously demonstrated the occurrence of DNA transfer of ARM genes ( nptII and aadA) from GM plant diet to blood cells and enteric microflora in rats.

The nutritional assessment of GMOs before commercialisation, how to approach a comprehensive assessment

Using the in vitro gas production technique, the fermentation kinetics of genetically modified (GM) corn and the gas production of GM soybean were respectively faster and lower compared to their respective conventional counterpart, although the chemical composition were not affect by the genetic modification.

Digestive fate of transgenic DNA and protein in livestock tissues fed genetically modified feed ingredients: A review

Findings from this study indicated that transgenic DNA is not completely degraded during feed processing and digestion and that DNA fragments could be detected by well-established detection methods such as PCR or qPCR in a number of organs and tissues of livestock animals.

Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal.

It is confirmed that feed-ingested DNA fragments (endogenous and transgenic) do survive to the terminal GI tract and that uptake into gut epithelial tissues does occur and a very low frequency of transmittance to visceral tissue was confirmed in pigs, but not in sheep.

Lack of detectable DNA uptake by bacterial gut isolates grown in vitro and by Acinetobacter baylyi colonizing rodents in vivo.

The authors' observations suggest the contents of the GIT shield or adsorb DNA, preventing detectable exposure of feed-derived DNA fragments to competent bacteria, is suggested to be responsible for the sharp 500- to 1,000,000-fold reduction of transformation frequencies seen.



Degradation of transgenic DNA from genetically modified soya and maize in human intestinal simulations.

The data indicate that some transgenes in GM foods may survive passage through the small intestine, and guar gum and tannic acid reduced the rate of DNA degradation throughout the small bowel simulations.

On the fate of orally ingested foreign DNA in mice: chromosomal association and placental transmission to the fetus

This distribution pattern argues for a transplacental pathway rather than for germline transmission which might be expected only after long-time feeding regimens, and in rare cells of three different fetuses, whose mothers have been fed with M13 DNA during gestation, the foreign DNA was detected by FISH in association with both chromatids.

Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent.

Only short term biological activity was observed in these environments, as shown by transformation to antibiotic resistance, and further studies are needed to elucidate the biological significance of free DNA in the rumen and oral cavities of sheep and in silage effluent.

The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material

Data indicated that only short DNA fragments derived from plant chloroplasts could be detected in the blood lymphocytes of cows and in all other cattle organs investigated, and no foreign plant DNA fragments were found in eggs.

Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice

The results of reconstitution experiments suggested that 2 to 4% of the orally administered M13 DNA could be detected in the GI tract of mice, and the possibility that the murine GI tract had been colonized by phage M13 was argued against.

The fate of antibiotic resistance marker genes in transgenic plant feed material fed to chickens.

The survival of the antibiotic resistance marker gene mirrored that of plant DNA targets, demonstrating that it survives no better than other DNA and indicating that it is very unlikely that bacteria in the gut of chickens will be transformed to ampicillin resistance when the birds are fed transgenic maize.

Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA.

Findings suggest transport of foreign DNA through the intestinal wall and Peyer's patches to peripheral blood leukocytes and into several organs in mice, suggesting medical and evolutionary implications of these observations may be considerable.

Application of molecular biological methods for studying probiotics and the gut flora

The future of molecular biology in the field of probiotics and the gut flora will, no doubt, stretch to investigations of functionality and activity of the microflora, and/or specific fractions.

Fate of Free DNA and Transformation of the Oral Bacterium Streptococcus gordonii DL1 by Plasmid DNA in Human Saliva

Findings indicate that DNA released from bacteria or food sources within the mouth has the potential to transform naturally competent oral bacteria, however, further investigations are needed to establish whether transformation of oral bacteria can occur at significant frequencies in vivo.

Effects of high-resistant-starch banana flour (RS(2)) on in vitro fermentation and the small-bowel excretion of energy, nutrients, and sterols: an ileostomy study.

The ileostomy model seems to give reliable results for in vivo measurement of RS, and in vitro fermentation of the ileal effluents obtained after the addition of RBF to the diet showed higher concentrations of acetate and butyrate.