Assessing the photoprotective effectiveness of non-photochemical chlorophyll fluorescence quenching: a new approach.

@article{Ruban2012AssessingTP,
  title={Assessing the photoprotective effectiveness of non-photochemical chlorophyll fluorescence quenching: a new approach.},
  author={Alexander V Ruban and Erik H. Murchie},
  journal={Biochimica et biophysica acta},
  year={2012},
  volume={1817 7},
  pages={977-82}
}
The photoprotective nature of non-photochemical quenching (NPQ) has not been effectively quantified and the major reason is the inability to quantitatively separate NPQ that acts directly to prevent photoinhibition of photosystem II (PSII). Here we describe a technique in which we use the values of the PSII yield and qP measured in the dark following illumination. We expressed the quantum yield of PSII (Φ(PSII)) via NPQ as: Φ(PSII)=qP×(Fv/Fo)/(1+Fv/Fo+NPQ). We then tested this theoretical… CONTINUE READING

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