Assessing the essentiality of the decaprenyl-phospho-d-arabinofuranose pathway in Mycobacterium tuberculosis using conditional mutants.

@article{Kolly2014AssessingTE,
  title={Assessing the essentiality of the decaprenyl-phospho-d-arabinofuranose pathway in Mycobacterium tuberculosis using conditional mutants.},
  author={Ga{\"e}lle S. Kolly and Francesca Boldrin and Claudia Sala and Neeraj Dhar and Ruben C. Hartkoorn and Marcello Ventura and Agnese Serafini and John D McKinney and Riccardo Manganelli and Stewart T Cole},
  journal={Molecular microbiology},
  year={2014},
  volume={92 1},
  pages={194-211}
}
In Mycobacterium tuberculosis the decaprenyl-phospho-d-arabinofuranose (DPA) pathway is a validated target for the drugs ethambutol and benzothiazinones. To identify other potential drug targets in the pathway, we generated conditional knock-down mutants of each gene involved using the TET-PIP OFF system. dprE1, dprE2, ubiA, prsA, rv2361c, tkt and rpiB were confirmed to be essential under non-permissive conditions, whereas rv3807c was not required for survival. In the most vulnerable group… CONTINUE READING

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