Assessing the effect of different ultrasonic frequencies on bacterial viability using flow cytometry.

@article{Joyce2011AssessingTE,
  title={Assessing the effect of different ultrasonic frequencies on bacterial viability using flow cytometry.},
  author={Elizabeth H. Joyce and Alaa G. Al-Hashimi and Timothy J. Mason},
  journal={Journal of applied microbiology},
  year={2011},
  volume={110 4},
  pages={862-70}
}
AIMS   This research investigated the effect of sonication at frequencies of 20, 40 and 580 kHz and approximately the same acoustic intensity on the viability and declumping of two micro-organisms (Escherichia coli and Klebsiella pneumonia). METHODS AND RESULTS   Two analytical methods were employed; viable plate counts (CFU ml−1) and flow cytometry to identify and quantify both live/viable and dead bacteria in the bulk liquid. Flow cytometry results for E. coli and Kl. pneumonia indicated a… CONTINUE READING
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