Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR

@inproceedings{Rutledge2010AssessingTP,
  title={Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR},
  author={R G Rutledge and Don Stewart},
  booktitle={PloS one},
  year={2010}
}
BACKGROUND Linear regression of efficiency or LRE introduced a new paradigm for conducting absolute quantification, which does not require standard curves, can generate absolute accuracies of +/-25% and has single molecule sensitivity. Derived from adapting the classic Boltzmann sigmoidal function to PCR, target quantity is calculated directly from the fluorescence readings within the central region of an amplification profile, generating 4-8 determinations from each amplification reaction… CONTINUE READING

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