Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material

Abstract

MicroRNAs (miRNAs) have emerged as promising cancer biomarkers. However, exploiting their informative potential requires careful optimization of their detection. Here, we compared the efficiency of commonly used RNA extraction kits in miRNA recovery from cells, plasma and urine/plasma-derived exosomes, using single-gene RT-qPCR and miRNA profiling. We used… (More)
DOI: 10.1038/srep19529

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Cite this paper

@inproceedings{ElKhoury2016AssessingCA, title={Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material}, author={Victoria El-Khoury and Sandrine Pierson and Tony Kaoma and François Bernardin and Guy J. Berchem}, booktitle={Scientific reports}, year={2016} }