Assessing Heterologous Expression of Hyoscyamine 6β-Hydroxylase – A Feasibility Study

@inproceedings{Averesch2014AssessingHE,
  title={Assessing Heterologous Expression of Hyoscyamine 6β-Hydroxylase – A Feasibility Study},
  author={Nils J H Averesch and Oliver Kayser},
  year={2014}
}
  • Nils J H Averesch, Oliver Kayser
  • Published 2014
  • Chemistry
  • Abstract The protein sequence of the hyoscyamine 6β-hydroxylase gene from Hyoscyamusniger was analysed in silico for its potential of heterologous expression. Therefore different parameters determining the proteins properties and structure in prokaryotic or eukaryotic protein expression systems were taken into account. In silico prediction of co- and post-translational modifications revealed 25 putative glycosylation sites, one of which reported to be a co-factor stabilizing residue in 2… CONTINUE READING

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