Assessing De Novo transcriptome assembly metrics for consistency and utility

  title={Assessing De Novo transcriptome assembly metrics for consistency and utility},
  author={Shawn T. O’Neil and Scott J. Emrich},
  booktitle={BMC Genomics},
Transcriptome sequencing and assembly represent a great resource for the study of non-model species, and many metrics have been used to evaluate and compare these assemblies. Unfortunately, it is still unclear which of these metrics accurately reflect assembly quality. We simulated sequencing transcripts of Drosophila melanogaster. By assembling these simulated reads using both a “perfect” and a modern transcriptome assembler while varying read length and sequencing depth, we evaluated quality… CONTINUE READING
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