Assembly of tomato blistering mosaic virus-like particles using a baculovirus expression vector system

@article{Vasques2019AssemblyOT,
  title={Assembly of tomato blistering mosaic virus-like particles using a baculovirus expression vector system},
  author={Raquel Medeiros Vasques and Roberto Franco Teixeira Corr{\^e}a and Leonardo A. da Silva and Rosana Blawid and Tatsuya Nagata and Bergmann Morais Ribeiro and Daniel M.P. Ardisson-Ara{\'u}jo},
  journal={Archives of Virology},
  year={2019},
  volume={164},
  pages={1753-1760}
}
The expression of several structural proteins from a wide variety of viruses in heterologous cell culture systems results in the formation of virus-like particles (VLPs). These VLPs structurally resemble the wild-type virus particles and have been used to study viral assembly process and as antigens for diagnosis and/or vaccine development. Tomato blistering mosaic virus (ToBMV) is a tymovirus that has a 6.3-kb positive-sense ssRNA genome. We have employed the baculovirus expression vector… 
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The effect of fusing the gene that encodes the surface antigen of hepatitis B virus (HBV) to either the N- or C-terminus of the AcMNPV Polh represents a promising tool for the production of reagents for the diagnosis of HBV infection.
Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds
TLDR
Current techniques used to target eukaryotic and prokaryotic species for the generation of correctly folded proteins with disulfide bonds are discussed.

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