Assembly of light-harvesting chlorophyll a/b complex in vitro. Time-resolved fluorescence measurements.

Abstract

The assembly kinetics of a pigment-binding membrane protein, the light- harvesting chlorophyll a/b complex of green plants, have been determined in vitro. Time-resolved fluorescence spectroscopy, with millisecond time resolution, has been used to monitor changes in both protein and chlorophyll (Chl) fluorescence, as well as in energy transfer from Chl b to… (More)

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