Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins.

@article{Trask2006AssemblyOH,
  title={Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins.},
  author={Shane D. Trask and Philip Ralph Dormitzer},
  journal={Journal of virology},
  year={2006},
  volume={80 22},
  pages={11293-304}
}
Assembly of the rotavirus outer capsid is the final step of a complex pathway. In vivo, the later steps include a maturational membrane penetration that is dependent on the scaffolding activity of a viral nonstructural protein. In vitro, simply adding the recombinant outer capsid proteins VP4 and VP7 to authentic double-layered rotavirus subviral particles (DLPs) in the presence of calcium and acidic pH increases infectivity by a factor of up to 10(7), yielding particles as infectious as… CONTINUE READING