Assembly of amber fragments of the beta subunit of Escherichia coli RNA polymerase.

@article{Gla1988AssemblyOA,
  title={Assembly of amber fragments of the beta subunit of Escherichia coli RNA polymerase.},
  author={Robert Gla\ss and N T Ralphs and Nobuyuki Fujita and Akira Ishihama},
  journal={European journal of biochemistry},
  year={1988},
  volume={176 2},
  pages={403-7}
}
Immunoblotting of size-separated whole cell proteins permitted the study of protein-protein interaction. Briefly, proteins obtained from cleared cell lysates of Escherichia coli were separated by glycerol gradient centrifugation and analysed by blotting against a set of specific antibodies. We have applied this procedure to the assembly of 11 N-terminal… CONTINUE READING