Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution

Abstract

A novel strategy to prepare negatively charged and small DNA-containing liposomes after condensation of plasmid DNA by a cationic lipid in deoxycholate micelle environment is described. The average diameter of resulting complexes was 62±8 nm. DNA-containing liposomes were then prepared by dialysis. The shape of the resulting liposomes was spherical. The average diameter and the surface charge of the liposomes were 86±6 nm and −24±3 mV, respectively. The plasmid DNA inside liposomes remained in a supercoiled form after incubation with DNase.

DOI: 10.1007/s10529-005-2734-5

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@article{Zhang2005AssemblyOP, title={Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution}, author={Hongwei Zhang and Ling Zhang and Xun Sun and Shu Diao and Zhirong Zhang}, journal={Biotechnology Letters}, year={2005}, volume={27}, pages={1701-1705} }