Assembly of New Individual Excitatory Synapses Time Course and Temporal Order of Synaptic Molecule Recruitment

@article{Friedman2000AssemblyON,
  title={Assembly of New Individual Excitatory Synapses Time Course and Temporal Order of Synaptic Molecule Recruitment},
  author={Hagit Friedman and Tal Bresler and C. Garner and N. Ziv},
  journal={Neuron},
  year={2000},
  volume={27},
  pages={57-69}
}
Time-lapse microscopy, retrospective immunohistochemistry, and cultured hippocampal neurons were used to determine the time frame of individual glutamatergic synapse assembly and the temporal order in which specific molecules accumulate at new synaptic junctions. New presynaptic boutons capable of activity-evoked vesicle recycling were observed to form within 30 min of initial axodendritic contact. Clusters of the presynaptic active zone protein Bassoon were present in all new boutons… Expand
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References

SHOWING 1-10 OF 121 REFERENCES
Potentiation of Evoked Vesicle Turnover at Individually Resolved Synaptic Boutons
TLDR
It is found that brief tetanic stimuli induce a potentiation of evoked synaptic vesicle turnover that lasts for at least 1 hr, and appears to occur approximately equally at boutons of low and high initial release probabilities, and evidently does not involve an increase in the size of the total recycling synapticvesicle pool. Expand
Assembly of presynaptic active zones from cytoplasmic transport packets
TLDR
Time-lapse fluorescence imaging, immunocytochemistry and electron microscopy are used to study hippocampal neuronal cultures transfected with a fusion construct of the presynaptic vesicle protein VAMP and green fluorescent protein to suggest that substantial membrane remodeling may be necessary to produce the uniform vesicles typical of the mature active zone. Expand
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticityExpand
Heterogeneity in the Molecular Composition of Excitatory Postsynaptic Sites during Development of Hippocampal Neurons in Culture
TLDR
The results suggest that synapse development proceeds by formation of a postsynaptic scaffold containing PSD-95 and GKAP in concert with presynaptic vesicle clustering, followed by regulated attachment of glutamate receptor subtypes to this scaffold. Expand
Dendritic spine changes associated with hippocampal long-term synaptic plasticity
TLDR
After induction of long-lasting (but not short-lasting) functional enhancement of synapses in area CA1, new spines appear on the postsynaptic dendrite, whereas in control regions on the same dendrites or in slices where long-term potentiation was blocked, no significant spine growth occurred. Expand
Dynamics of Synaptic Vesicles in Cultured Spinal Cord Neurons in Relationship to Synaptogenesis
  • Z. Dai, H. Peng
  • Medicine, Biology
  • Molecular and Cellular Neuroscience
  • 1996
TLDR
The dynamics of synaptic vesicles during the development of presynaptic specializations in cultured Xenopus spinal cord neurons was studied with the fluorescent vesicular probe FM1-43 and suggested that SVs become localized at the target by interacting with an actin-based cytoskeletal specialization in a phosphorylation-sensitive manner. Expand
The kinetics of synaptic vesicle recycling measured at single presynaptic boutons
TLDR
It is shown that endocytosis is not dependent on membrane potential and, unlike exocytotic, that it is independent of extracellular Ca2+. Expand
The Development of Excitatory Synapses in Cultured Spinal Neurons
TLDR
Using cultured rat spinal cord neurons, it is demonstrated that clustering of the AMPA receptor subunit GluR1 is among the earliest events in excitatory synapse formation in vitro, coincident with the onset of miniature EPSCs and in many cases preceding presynaptic vesicle accumulation. Expand
Evidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation
TLDR
It is suggested that dendritic filopodia may actively initiate synaptogenic contacts with nearby axons and thereafter evolve into dendrite spines. Expand
Hippocampal synaptogenesis in cell culture: developmental time course of synapse formation, calcium influx, and synaptic protein distribution
TLDR
Data suggest that the expression of functional omega- CgTx-sensitive calcium influx is temporally coincident with synapse formation, and that during the maturation of the synapse there is a redistribution of synaptic proteins. Expand
...
1
2
3
4
5
...