Assembly of Escherichia coli 50 S ribosomes from ribonucleic acid and protein components. I. Chemical and physical factors affecting the conformation of assembled particles.


The in vitro assembly of Escherichia coli 50 S ribosomes from the ribonucleic acid and protein components of 50 S ribosomes was attempted. The two components were prepared by subjecting 50 S ribosomes to LiCl-urea treatment. The ribonucleic acid fraction contains 23 S RNA and 5 S RNA in equimolar amounts and is free from significant amounts of protein. The… (More)


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