Assembly of ER-associated protein degradation in vitro: dependence on cytosol, calnexin, and ATP

Abstract

To investigate the mechanisms of ER-associated protein degradation (ERAD), this process was reconstituted in vitro. Established procedures for post-translational translocation of radiolabeled prepro-alpha factor into isolated yeast microsomes were modified to inhibit glycosylation and to include a posttranslocation "chase" incubation period to monitor… (More)

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