Assembly of Designer TAL Effectors by Golden Gate Cloning

@inproceedings{Weber2011AssemblyOD,
  title={Assembly of Designer TAL Effectors by Golden Gate Cloning},
  author={Ernst Weber and Ramona Gruetzner and Stefan Werner and Carola Engler and Sylvestre Marillonnet},
  booktitle={PloS one},
  year={2011}
}
Generation of customized DNA binding domains targeting unique sequences in complex genomes is crucial for many biotechnological applications. The recently described DNA binding domain of the transcription activator-like effectors (TALEs) from Xanthomonas consists of a series of repeats arranged in tandem, each repeat binding a nucleotide of the target sequence. We present here a strategy for engineering of TALE proteins with novel DNA binding specificities based on the 17.5 repeat-containing… CONTINUE READING

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