• DOI:10.1038/s41467-017-01875-9
Corpus ID: 23058354

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

@article{CarlsonStevermer2017AssemblyOC,
  title={Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing},
  author={Jared Carlson-Stevermer and Amr A Abdeen and Lucille Kohlenberg and Madelyn Goedland and Kaivalya Molugu and Meng Lou and K. Saha},
  journal={Nature Communications},
  year={2017},
  volume={8}
}

Jared Carlson-Stevermer, Amr A Abdeen, +4 authors K. Saha

Published 2017

Biology, Medicine

Nature Communications

Writing specific DNA sequences into the human genome is challenging with non-viral gene-editing reagents, since most of the edited sequences contain various imprecise insertions or deletions. We developed a modular RNA aptamer-streptavidin strategy, termed S1mplex, to complex CRISPR-Cas9 ribonucleoproteins with a nucleic acid donor template, as well as other biotinylated molecules such as quantum dots. In human cells, tailored S1mplexes increase the ratio of precisely edited to imprecisely… CONTINUE READING

View on Springer

nature.com

Save to Library

Create Alert

Cite

Launch Research Feed