Assembly of 18-nucleotide primers by ligation of three hexamers: sequencing of large genomes by primer walking.

Abstract

A novel method (SPEL-6) for sequencing large genomes permits 10-min synthesis of 18-mer primers and their immediate use in DNA sequencing by primer walking. Primers are ligated from three contiguous hexamers complementary to the single-stranded or denatured DNA (150:1 ratio), which is used both as the template for ligation and as the DNA to be sequenced… (More)

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