Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: the E1 glycoprotein of coronavirus mouse hepatitis virus A59.

@article{Rottier1984AssemblyIV,
  title={Assembly in vitro of a spanning membrane protein of the endoplasmic reticulum: the E1 glycoprotein of coronavirus mouse hepatitis virus A59.},
  author={Peter Rottier and Dietrich Brandenburg and John H Armstrong and Bernard A.M. van der Zeijst and Graham Warren},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 5},
  pages={
          1421-5
        }
}
The E1 glycoprotein of coronavirus mouse hepatitis virus A59 was synthesized in vitro by translation of viral mRNA in the presence of dog pancreatic microsomes. Its disposition in the membrane was investigated by digestion with proteases and by selective NH2-terminal labeling. The protein spans the membrane, but only small portions from the NH2 and COOH terminus are exposed respectively in the lumenal and cytoplasmic domains; the bulk of the molecule is apparently buried in the membrane. The… CONTINUE READING
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