Assembly-dependent phosphorylation of myosin and paramyosin of native thick filaments in Caenorhabditis elegans.

@article{Dey1992AssemblydependentPO,
  title={Assembly-dependent phosphorylation of myosin and paramyosin of native thick filaments in Caenorhabditis elegans.},
  author={Chinmoy S. Dey and Philip R. Deitiker and Henry F. Epstein},
  journal={Biochemical and biophysical research communications},
  year={1992},
  volume={186 3},
  pages={1528-32}
}
Phosphorylation of the thick filament proteins myosin and paramyosin was studied in Caenorhabditis elegans. We have incubated partially purified, native thick filaments with [gamma 32P] ATP in the presence of 50-750 mM NaCl, pH 6.5-8.0. Myosin heavy chain and paramyosin were phosphorylatable only upon solubilization at 450 mM and higher NaCl concentrations… CONTINUE READING