Assembly and transcription of synthetic vesicular stomatitis virus nucleocapsids.

  title={Assembly and transcription of synthetic vesicular stomatitis virus nucleocapsids.},
  author={Sue A. Moyer and Sherin Smallwood-Kentro and Abraham Haddad and Ludvik Prevec},
  journal={Journal of virology},
  volume={65 5},
The functional template for transcription of vesicular stomatitis virus (VSV) RNA is a ribonucleoprotein particle (nucleocapsid) consisting of the negative-strand sense genomic RNA completely encapsidated by the viral nucleocapsid (N) protein. As an approach to create nucleocapsids in vitro, we demonstrate here the specific encapsidation by purified N protein of in vitro-synthesized RNA sequences representing the 5' end of both the negative- and positive-strand VSV genome-length RNAs. As few as… CONTINUE READING
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