Assembly and trafficking of caveolar domains in the cell

  title={Assembly and trafficking of caveolar domains in the cell},
  author={Akiko Tagawa and Anna Mezzacasa and Arnold Hayer and Andrea Longatti and Lucas Pelkmans and Ari Helenius},
  journal={The Journal of Cell Biology},
  pages={769 - 779}
Using total internal reflection fluorescence microscopy (TIR-FM), fluorescence recovery after photobleaching (FRAP), and other light microscopy techniques, we analyzed the dynamics, the activation, and the assembly of caveolae labeled with fluorescently tagged caveolin-1 (Cav1). We found that when activated by simian virus 40 (SV40), a non-enveloped DNA virus that uses caveolae for cell entry, the fraction of mobile caveolae was dramatically enhanced both in the plasma membrane (PM) and in the… CONTINUE READING
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