Assembly and sealing of tight junctions: Possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin

  title={Assembly and sealing of tight junctions: Possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin},
  author={Maria Susana Balda and Lorenza Gonz{\'a}lez-Mariscal and Rub{\'e}n Gerardo Contreras and Marina Mac{\'i}as-Silva and Mar{\'i}a Eugenia Torres-M{\'a}rquez and J. A. Garc{\'i}a S{\'a}inz and Marcelino Cereijido},
  journal={The Journal of Membrane Biology},
The making and sealing of a tight junction (TJ) requires cell-cell contacts and Ca2−, and can be gauged through the development of transepithelial electrical resistance (TER) and the accumulation of ZO-1 peptide at the cell borders. We observe that pertussis toxin increases TER, while AIF3 and carbamil choline (carbachol) inhibit it, and 5-guanylylimidodiphosphate (GTPΓs) blocks the development of a cell border pattern of ZO-1, suggesting that G-proteins are involved. Phospholipase C (PLC) and… CONTINUE READING


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