Assembly and function of the archaeal flagellum.
@article{Ghosh2011AssemblyAF, title={Assembly and function of the archaeal flagellum.}, author={Abhrajyoti Ghosh and Sonja-Verena Albers}, journal={Biochemical Society transactions}, year={2011}, volume={39 1}, pages={ 64-9 } }
Motility is a common behaviour in prokaryotes. [] Key Result Recent computational analysis in our laboratory has identified conserved highly charged loop regions within one of the core proteins of the flagellum, the membrane integral protein FlaJ, and predicted that these are involved in the interaction with the assembly ATPase FlaI. Interestingly, considerable variation was found among the loops of FlaJ from the two major subkingdoms of archaea, the Euryarchaeota and the Crenarchaeota. Understanding the…
62 Citations
A comprehensive history of motility and Archaellation in Archaea
- Biology, History
- 2021
A historical overview on archaella and motility research in Archaea is provided, beginning with the first simple observations of motile extreme halophilic archaea a century ago up to state-of-the-art cryo-tomography of the archaellum motor complex and filament observed today.
Archaeal flagellar ATPase motor shows ATP-dependent hexameric assembly and activity stimulation by specific lipid binding.
- BiologyThe Biochemical journal
- 2011
The results of the present study report the first detailed biochemical analyses of the motor protein of an archaeal flagellum, and show that purified FlaI is a Mn2+-dependent ATPase that binds MANT-ATP with a high affinity and hydrolyses ATP in a co-operative manner.
Molecular analysis of the crenarchaeal flagellum
- BiologyMolecular microbiology
- 2012
All seven fla genes are essential for crenarchaeal flagellum assembly and function, and it is demonstrated that lack of motility coincided with the loss of flagella assembly.
Insights into subunit interactions in the Sulfolobus acidocaldarius archaellum cytoplasmic complex
- BiologyThe FEBS journal
- 2013
This study shows by biochemical and biophysical techniques that FlaX from S. acidocaldarius acts as a cytoplasmic scaffold in archaellum assembly, as it interacts with FlaI as well as with the recA family protein FlaH, the only cytopLasmic components of the archaellsum.
Archaeal type IV pili and their involvement in biofilm formation
- BiologyFront. Microbiol.
- 2015
Recent advances in the understanding of the functional roles played by archaeal type IV adhesion pili and their subunits are discussed, with particular emphasis on their involvement in biofilm formation.
FlaX, A Unique Component of the Crenarchaeal Archaellum, Forms Oligomeric Ring-shaped Structures and Interacts with the Motor ATPase FlaI
- BiologyThe Journal of Biological Chemistry
- 2012
FlaX, a conserved subunit in crenarchaeal archaella, formed high molecular weight complexes that adapted a ring-like structure with an approximate diameter of 30 nm that was not only involved in the oligomerization, but also essential for FlaX interaction with FlaI, the bifunctional ATPase that is involved in assembly and rotation of the archaellum.
Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis
- BiologyEMBO reports
- 2017
Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of a…
The archaellum: a rotating type IV pilus
- BiologyMolecular microbiology
- 2014
A novel type of behaviour for type IV pilus like structures: archaella rotate and their rotation drives swimming motility is revealed, and it is demonstrated that temperature has a direct effect on rotation velocity explaining temperature‐dependent swimming velocity.
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
- BiologyLife
- 2016
This study identifies 20 genes, previously unknown to affect motility or adhesion, and underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects.
Molecular dynamics simulation of bacterial flagella
- BiologyBiophysical Reviews
- 2017
Molecular dynamics simulation is a powerful method that permits the investigation, at atomic resolution, of the molecular mechanisms of biomolecular systems containing many proteins and solvent and when applied to the flagellum, these studies successfully unveiled the polymorphic supercoiling and transportation mechanism of the filament.
References
SHOWING 1-10 OF 57 REFERENCES
Archaeal Flagella, Bacterial Flagella and Type IV Pili: A Comparison of Genes and Posttranslational Modifications
- BiologyJournal of Molecular Microbiology and Biotechnology
- 2006
It has been suggested that the archaeal flagellum and the bacterial type IV pilus share a similar mode of assembly, and N-linked glycans have recently been reported on Archaeal Flagellins, both consistent with the proposed assembly model.
Phylogenomics of the archaeal flagellum: rare horizontal gene transfer in a unique motility structure
- BiologyBMC Evolutionary Biology
- 2007
It is shown that the components of the archaeal flagellar system have not been frequently transferred among archaealing species, indicating that gene fixation following HGT can also be rare for genes encoding components of large macromolecular complexes with a structural role.
The Archaeabacterial Flagellar Filament: A Bacterial Propeller with a Pilus-Like Structure
- BiologyJournal of Molecular Microbiology and Biotechnology
- 2006
The archaeabacterial filament is glycosylated, which is uncommon in eubacterial flagella and is believed to be one of the key elements for stabilizing proteins under extreme conditions, and shares structural and functional similarities with type IV pili and eub bacterial flagellar filaments, correspondingly.
Systematic deletion analyses of the fla genes in the flagella operon identify several genes essential for proper assembly and function of flagella in the archaeon, Methanococcus maripaludis
- BiologyMolecular microbiology
- 2007
Results demonstrate for first time that these fla operon genes are directly involved and critically required for proper archaeal flagella assembly and function.
The flagellar filament structure of the extreme acidothermophile Sulfolobus shibatae B12 suggests that archaeabacterial flagella have a unique and common symmetry and design.
- BiologyJournal of molecular biology
- 2008
Mutants in flaI and flaJ of the archaeon Methanococcus voltae are deficient in flagellum assembly
- BiologyMolecular microbiology
- 2002
The fla− phenotype and protein secretion characteristics of the flaI and flaJ mutants therefore implicate these respective genes in archaeal flagellin secretion and assembly, and further support a model describing the ArchaealFlagellum as a novel prokaryotic motility structure.
Cleavage of preflagellins by an aspartic acid signal peptidase is essential for flagellation in the archaeon Methanococcus voltae
- BiologyMolecular microbiology
- 2003
FlaK of Methanococcus voltae retains its preflagellin peptidase activity when expressed in Escherichia coli and used in an in vitro assay, which further emphasizes the similarity archaeal flagella have with type IV pili, rather than with bacterial flagellins.
How bacteria assemble flagella.
- BiologyAnnual review of microbiology
- 2003
The bacterial flagellum is both a motor organelle and a protein export/assembly apparatus that extends from the cytoplasm to the cell exterior and employs a type III pathway, utilized for secretion of virulence factors.
The archaeal flagellum: a unique motility structure
- BiologyJournal of bacteriology
- 1996
The phylogenetic structure of the procaryotic domain: the primary kingdoms of Archaea, Bacteria and Eucarya is studied.
Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella.
- BiologyMicrobiology
- 2003
Homologues of the protein constituents of the Klebsiella pneumoniae (Klebsiella oxytoca) type II secreton (T2S), the Pseudomonas aeruginosa type IV pilus/fimbrium biogenesis machinery (T4P) and the…