Assembly and function of the archaeal flagellum.

@article{Ghosh2011AssemblyAF,
  title={Assembly and function of the archaeal flagellum.},
  author={Abhrajyoti Ghosh and Sonja-Verena Albers},
  journal={Biochemical Society transactions},
  year={2011},
  volume={39 1},
  pages={
          64-9
        }
}
Motility is a common behaviour in prokaryotes. [] Key Result Recent computational analysis in our laboratory has identified conserved highly charged loop regions within one of the core proteins of the flagellum, the membrane integral protein FlaJ, and predicted that these are involved in the interaction with the assembly ATPase FlaI. Interestingly, considerable variation was found among the loops of FlaJ from the two major subkingdoms of archaea, the Euryarchaeota and the Crenarchaeota. Understanding the…
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A comprehensive history of motility and Archaellation in Archaea
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A historical overview on archaella and motility research in Archaea is provided, beginning with the first simple observations of motile extreme halophilic archaea a century ago up to state-of-the-art cryo-tomography of the archaellum motor complex and filament observed today.
Archaeal flagellar ATPase motor shows ATP-dependent hexameric assembly and activity stimulation by specific lipid binding.
TLDR
The results of the present study report the first detailed biochemical analyses of the motor protein of an archaeal flagellum, and show that purified FlaI is a Mn2+-dependent ATPase that binds MANT-ATP with a high affinity and hydrolyses ATP in a co-operative manner.
Molecular analysis of the crenarchaeal flagellum
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All seven fla genes are essential for crenarchaeal flagellum assembly and function, and it is demonstrated that lack of motility coincided with the loss of flagella assembly.
Insights into subunit interactions in the Sulfolobus acidocaldarius archaellum cytoplasmic complex
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This study shows by biochemical and biophysical techniques that FlaX from S. acidocaldarius acts as a cytoplasmic scaffold in archaellum assembly, as it interacts with FlaI as well as with the recA family protein FlaH, the only cytopLasmic components of the archaellsum.
Archaeal type IV pili and their involvement in biofilm formation
TLDR
Recent advances in the understanding of the functional roles played by archaeal type IV adhesion pili and their subunits are discussed, with particular emphasis on their involvement in biofilm formation.
FlaX, A Unique Component of the Crenarchaeal Archaellum, Forms Oligomeric Ring-shaped Structures and Interacts with the Motor ATPase FlaI
TLDR
FlaX, a conserved subunit in crenarchaeal archaella, formed high molecular weight complexes that adapted a ring-like structure with an approximate diameter of 30 nm that was not only involved in the oligomerization, but also essential for FlaX interaction with FlaI, the bifunctional ATPase that is involved in assembly and rotation of the archaellum.
Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis
Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of a…
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A novel type of behaviour for type IV pilus like structures: archaella rotate and their rotation drives swimming motility is revealed, and it is demonstrated that temperature has a direct effect on rotation velocity explaining temperature‐dependent swimming velocity.
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants
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This study identifies 20 genes, previously unknown to affect motility or adhesion, and underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects.
Molecular dynamics simulation of bacterial flagella
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Molecular dynamics simulation is a powerful method that permits the investigation, at atomic resolution, of the molecular mechanisms of biomolecular systems containing many proteins and solvent and when applied to the flagellum, these studies successfully unveiled the polymorphic supercoiling and transportation mechanism of the filament.
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