Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures

@article{Angelides1989AssemblyAE,
  title={Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures},
  author={Kimon J. Angelides and Kelvin E Smith and Masashi Takeda},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={108},
  pages={1495 - 1506}
}
We have explored the dynamics of intermediate filament assembly and subunit exchange using fluorescently labeled neurofilament proteins and a fluorescence resonance energy transfer assay. Neurofilaments (NFs) are assembled from three highly phosphorylated proteins with molecular masses of 180 (NF-H), 130 (NF-M), and 66 kD (NF-L) of which NF-L forms the… CONTINUE READING