Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing.

Abstract

De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasopharyngeal swabs in the context of the microbial community. Data were generated with a prior sequence independent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full… (More)

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@article{Bartolini2011AssemblyAC, title={Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing.}, author={Barbara Bartolini and Giovanni Chillemi and Isabella Abbate and Alessandro Bruselles and Gabriella Rozera and Tiziana Castrignan{\`o} and Daniele Paoletti and Ernesto Picardi and Alessandro Desideri and Graziano Pesole and M. Capobianchi}, journal={The new microbiologica}, year={2011}, volume={34 4}, pages={391-7} }