Assembling Glycan-Charged Dolichol Phosphates: Chemoenzymatic Synthesis of a Haloferax volcanii N-Glycosylation Pathway Intermediate.

@article{Elharar2017AssemblingGD,
  title={Assembling Glycan-Charged Dolichol Phosphates: Chemoenzymatic Synthesis of a Haloferax volcanii N-Glycosylation Pathway Intermediate.},
  author={Yifat Elharar and Ananda Rao Podilapu and Ziqiang Guan and Suvarn S Kulkarni and Jerry Eichler},
  journal={Bioconjugate chemistry},
  year={2017},
  volume={28 9},
  pages={2461-2470}
}
N-glycosylation, the covalent attachment of glycans to select protein target Asn residues, is a post-translational modification performed by all three domains of life. In the halophilic archaea Haloferax volcanii, in which understanding of this universal protein-processing event is relatively well-advanced, genes encoding the components of the archaeal glycosylation (Agl) pathway responsible for the assembly and attachment of an N-linked pentasaccharide have been identified. As elsewhere, the N… CONTINUE READING