Assaying the kinetics of protein denaturation catalyzed by AAA+ unfolding machines and proteases.

@article{Baytshtok2015AssayingTK,
  title={Assaying the kinetics of protein denaturation catalyzed by AAA+ unfolding machines and proteases.},
  author={Vladimir Baytshtok and Tania A. Baker and Robert T. Sauer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2015},
  volume={112 17},
  pages={5377-82}
}
ATP-dependent molecular machines of the AAA+ superfamily unfold or remodel proteins in all cells. For example, AAA+ ClpX and ClpA hexamers collaborate with the self-compartmentalized ClpP peptidase to unfold and degrade specific proteins in bacteria and some eukaryotic organelles. Although degradation assays are straightforward, robust methods to assay the… CONTINUE READING