Assaying chromosome pairing by FISH analysis of spread Saccharomyces cerevisiae nuclei.


Fluorescent in situ hybridization (FISH) provides a powerful tool to study the localization of DNA sequences in relationship to one another. FISH has the advantage over other methods, notably use of GFP-tagged repressor/operator arrays, that an almost unlimited number of probes can be utilized without having to make new strains for each new locus one wants… (More)
DOI: 10.1007/978-1-60761-103-5_3


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