Assaying chemotaxis of Dictyostelium cells.

@article{Mendoza2006AssayingCO,
  title={Assaying chemotaxis of Dictyostelium cells.},
  author={Michelle C. Mendoza and Richard A. Firtel},
  journal={Methods in molecular biology},
  year={2006},
  volume={346},
  pages={393-405}
}
Both prokaryote and eukaryote cells can sense and move up chemical concentration gradients (chemotax). As a means of finding food sources during vegetative growth, Dictyostelium discoideum naturally chemotaxes toward chemicals released by bacteria. As part of its developmental life cycle, D. discoideum chemotaxes towards cAMP. This chapter describes protocols for using Dictyostelium to understand the cell biology behind and the signaling events necessary for eukaryotic amoeboid chemotaxis. The… CONTINUE READING