Assaying P-Type ATPases Reconstituted in Liposomes.

@article{Apell2016AssayingPA,
  title={Assaying P-Type ATPases Reconstituted in Liposomes.},
  author={H. Apell and Bojana Damnjanovi{\'c}},
  journal={Methods in molecular biology},
  year={2016},
  volume={1377},
  pages={
          127-56
        }
}
Reconstitution of P-type ATPases in unilamellar liposomes is a useful technique to study functional properties of these active ion transporters. Experiments with such liposomes provide an easy access to substrate-binding affinities of the ion pumps as well as to the lipid and temperature dependence of the pump current. Here, we describe two reconstitution methods by dialysis and the use of potential-sensitive fluorescence dyes to study transport properties of two P-type ATPases, the Na,K-ATPase… Expand
Mechanistic principles of ion transport in the Na,K-ATPase
  • H. Apell
  • Chemistry
  • Russian Journal of Electrochemistry
  • 2017
The Na,K-ATPase is a member of the P-type ATPase family and a primary active ion transporter for Na+ and K+ ions in the cytoplasmic membrane of virtually all animal cells. Considerable progress inExpand
The KdpFABC complex – K+ transport against all odds
TLDR
Evidence supporting contradictory models of the KdpFABC complex is discussed and key experiments are identified needed to resolve discrepancies and produce a unified model for this fascinating mechanistic hybrid. Expand

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