Assay optimization and kinetic profile of the human and the rabbit isoforms of 11beta-HSD1.

Abstract

Assay conditions for the 11beta-hydroxysteroid dehydrogenase have been optimized by adding phospholipids in the media buffer to increase and stabilize the enzymatic activity. The presence of phospholipids greatly facilitates the study of the binding of cortisone and NADPH at the enzyme catalytic site. Kinetic analyses conducted with the human and rabbit… (More)

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@article{Castro2007AssayOA, title={Assay optimization and kinetic profile of the human and the rabbit isoforms of 11beta-HSD1.}, author={Arturo Castro and Jeff X. Zhu and Gordon Alton and Paul A. Rejto and Jacques Ermolieff}, journal={Biochemical and biophysical research communications}, year={2007}, volume={357 2}, pages={561-6} }