Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase.

@article{Bozeman1990AssayOT,
  title={Assay of the human leukocyte enzymes myeloperoxidase and eosinophil peroxidase.},
  author={Paula M. Bozeman and Douglas B. Learn and E. Llewellyn Thomas},
  journal={Journal of immunological methods},
  year={1990},
  volume={126 1},
  pages={
          125-33
        }
}
Conditions were optimized for measuring the activity of myeloperoxidase (MPO) and the eosinophil peroxidase (EPO) with tetramethylbenzidine (TMB) as the substrate. Detergents caused a small increase in the measured activity of the purified enzymes and were required when isolated neutrophils or eosinophils were assayed. Sharp concentration optima were observed with both ionic and non-ionic detergents. Activity was also influenced by halide ions. Bromide or iodide caused up to a 7-fold increase… CONTINUE READING
BETA

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 45 CITATIONS

Development of a colorimetric method for the dosage of OI- anions and I2 in aqueous media.

  • Communications in agricultural and applied biological sciences
  • 2014
VIEW 5 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Neutrophil extracellular traps - the dark side of neutrophils.

  • The Journal of clinical investigation
  • 2016
VIEW 1 EXCERPT
CITES BACKGROUND

Strain differences in toxicity of oral cadmium intake in rats.

  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2016
VIEW 1 EXCERPT
CITES METHODS