Assay of the concentration and 13C-labeling pattern of phenylacetylglutamine by nuclear magnetic resonance.

@article{Dugelay1994AssayOT,
  title={Assay of the concentration and 13C-labeling pattern of phenylacetylglutamine by nuclear magnetic resonance.},
  author={Sam Dugelay and Dawei Yang and Maxim V Soloviev and Stephen F. Previs and Kadambri Agarwal and Carolyn A. Fernandez and Henri Brunengraber},
  journal={Analytical biochemistry},
  year={1994},
  volume={221 2},
  pages={368-73}
}
Phenylacetate, derived from phenylalanine, is converted in human and primate liver to phenylacetylglutamine. The latter, which is excreted in urine, has been used to probe noninvasively the labeling pattern of liver citric acid cycle intermediates. We present nuclear magnetic resonance assays for the urinary concentration of phenylacetylglutamine and for the 13C-labeling pattern of its glutamine moiety. The concentration of phenylacetylglutamine is calculated from the natural 13C signals of all… CONTINUE READING