Assay of metoprolol and alpha-hydroxymetoprolol in human urine by reversed-phase liquid chromatography with direct-injection.

AIM To develop an HPLC method with direct injection for the simultaneous determination of metoprolol (M) and alpha-hydroxymetoprolol (HM) in human urine. METHODS Urine (200 microliters) was diluted with eluate and injected into the chromatograph. Samples were separated on an ODS column by isocratic binary elution and monitored by fluorescence detection… CONTINUE READING