Assay of malondialdehyde in biological fluids by gas chromatography-mass spectrometry.

  title={Assay of malondialdehyde in biological fluids by gas chromatography-mass spectrometry.},
  author={H. C. Yeo and H J Helbock and D W Chyu and Bruce N. Ames},
  journal={Analytical biochemistry},
  volume={220 2},
Malondialdehyde (MDA) is assayed in femtomole quantities in biological samples by gas chromatography-mass spectrometry (GC-MS). The MDA trapped in protein as a Schiff base is released by H2SO4, the protein precipitated using Na2WO4, and the MDA derivatized with pentafluorophenylhydrazine to form the stable adduct, N-pentafluorophenylpyrazole. Negative chemical ionization (NCI) capability allows the sensitive detection of this MDA adduct in biological samples at a level of 5 nM on-column. A… CONTINUE READING


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