Assay of fluconazole by high-performance liquid chromatography with a mixed-phase column.

@article{Wallace1992AssayOF,
  title={Assay of fluconazole by high-performance liquid chromatography with a mixed-phase column.},
  author={J. E. Wallace and Steven C. Harris and Jessica Gallegos and Glenn Foulds and Tong J Chen and Michael G. Rinaldi},
  journal={Antimicrobial agents and chemotherapy},
  year={1992},
  volume={36 3},
  pages={603-6}
}
A mixed-phase liquid chromatographic column was used to assay fluconazole in plasma, serum, and cerebrospinal fluid. The assay was linear from 0.2 to 20 micrograms/ml, with an average coefficient of variation of less than 5%. The partitioning of the drug between serum and cerebrospinal fluid was determined for 34 patients. The method was demonstrated to be suitable for both pharmacokinetic studies and monitoring of patients receiving treatment with this antifungal agent.