Assay of cyclooxygenase-1 and 2 in human monocytes

  title={Assay of cyclooxygenase-1 and 2 in human monocytes},
  author={Maryanne Demasi and Gillian Caughey and Michael John James and Leslie G. Cleland},
  journal={Inflammation Research},
Objective and Design: There is frequently poor correlation between in vitro methods for calculated cyclooxygenase (COX)-1/COX-2 selectivities of inflammatory agents. Therefore, we have examined the use of a single stimulus in a single cell type containing both COX isoforms, for determining the selectivities of COX-inhibitory agents.¶Methods: Fresh human monocytes were stimulated with arachidonic acid (AA; 10 μM) for 15min and prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) production were… CONTINUE READING


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