Assay of chlortetracycline in animal feeds by liquid chromatography with fluorescence detection.


A liquid chromatographic (LC) method is described to assay chlortetracycline (CTC) at gram-per-ton and gram-per-pound levels. Feeds and premixes are extracted with acid-acetone following AOAC Method 957.23B(h). Extracts are filtered and quantitated by LC using a C18 column and fluorescence detection at 390 nm excitation and 512 nm emission. The mobile phase… (More)


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