Assay of angiotensin I-converting enzyme-inhibiting activity based on the detection of 3-hydroxybutyric acid.

@article{Lam2007AssayOA,
  title={Assay of angiotensin I-converting enzyme-inhibiting activity based on the detection of 3-hydroxybutyric acid.},
  author={Le Hoang Lam and Tomoko Shimamura and Ken Sakaguchi and Katsuya Noguchi and Munetaka Ishiyama and Yume Fujimura and Hiroyuki Ukeda},
  journal={Analytical biochemistry},
  year={2007},
  volume={364 2},
  pages={104-11}
}
Hypertension and related diseases afflict millions of individuals worldwide, and many investigations of angiotensin I-converting enzyme (ACE) activity have been carried out. Most of these have used hippuryl-histidyl-leucine (HHL) as a substrate for ACE reaction with considerable interferences. Here we propose the use of a new substrate, 3-hydroxybutyrylglycyl-glycyl-glycine (3HB-GGG) for the screening of ACE inhibitors. Under the actions of ACE and aminoacylase, 3HB-GGG is cleaved into amino… CONTINUE READING

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